DNA barcoding against poaching of Chamois (Rupicapra rupicapra), two confirmed cases from Greece

Document Type: Short communication

Authors

1 Molecular Ecology and Conservation Genetics Lab, Department of Biological Applications and Technology, University of Ioannina, 45500, Ioannina, Greece

2 Molecular Ecology and Conservation Genetics Lab, Department of Biological Applications and Technology, University of Ioannina, 45110, Ioannina, Greece

Abstract

Poaching has been reported as a major threat to the survival of many species including the Chamois (Rupicapra rupicapra). We report two recent cases from Greece where COI and Cytb sequences were used for species identification from evidence samples. Not only we confirm the poaching events, but through the description of the laboratory procedures we highlight two limitations of the barcoding method, COI-like sequences and the universality of COI primers.

Keywords


Allendorf F.W., England P.R., Luikart G., Ritchie P.A., Ryman N. 2008. Genetic effects of harvest on wild animal populations. Trends in Ecology and Evolution 23(6):327-337.

Buhay J.E. 2009. “COI-like” sequences are becoming problematic in molecular systematic and DNA barcoding studies. Journal of Crustacean Biology 29(1):96-110.

Corlatti L., Lorenzini R., Lovari S. 2011. The conservation of the Chamois Rupicapra spp. Mammal Review 41(2):163-174.

Folmer O., Black M., Hoeh W., Lutz R., Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular marine biology and biotechnology 3(5):294.

Gavin M.C., Solomon J.N., Blank S.G. 2010. Measuring and monitoring illegal use of natural resources. Conservation Biology 24(1):89-100.

Hebert P.D., Cywinska A., Ball S.L. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences 270(1512):313-321.

Kocher T.D., Thomas W.K., Meyer A., Edwards S.V., Pääbo S., Villablanca F.X., Wilson A.C. 1989. Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences 86(16):6196-6200.

Kumar S., Stecher G., Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7):1870-1874.

Linacre A., Tobe S.S. 2011. An overview to the investigative approach to species testing in wildlife forensic science. Investigative genetics 2:2.

Papaioannou H.I., Kati V.I. 2007. Current status of the Balkan Chamois (Rupicapra rupicapra balcanica) in Greece: Implications for conservation. Belgian Journal of Zoology 137(1):33-39.

Papaioannou H., Sgardelis S., Chondropoulos B., Vassilakis D., Kati V., Dimopoulos P. 2015. Demographic characteristics, seasonal range and habitat topography of Balkan Chamois population in its southernmost limit of its distribution (Giona mountain, Greece). Journal of Natural History 49(5-8):327-345.

Pérez T., Rodríguez F., Fernández M., Albornoz J., Domínguez A. 2017. Ancient mitochondrial pseudogenes reveal hybridization between distant lineages in the evolution of the Rupicapra genus. Gene 628:63-71.

Verma S., Singh L. 2003. Novel universal primers establish identity of an enormous number of animal species for forensic application. Molecular Ecology Resources 3(1):28-31.